The Wayback Machine - https://web.archive.org/web/20210424184519/https://www.bartleby.com/essay/Enzyme-Activity-F3P9Y7RAZ9EW

Enzyme Activity

1327 Words6 Pages
Manuel Mark Guerrero

3/12/13

Biology 1010c

Mrs.Bisogno

Title:

What is the effect of temperature, pH, and substrate concentration on Enzyme activity?

Purpose:

Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzymes have only one substrate that breaks down. Enzymes are produced in the cells of the body and affect the rate of almost all the chemical reactions which take place in living organisms. The rate of enzymes activity is influenced by temperature, pH, and substrate concentration. The purpose of this lab was to determine the affects of enzyme activity under specific temperature changes, pH values and substrate concentration. Since heat
…show more content…
4. Wait 5 minutes and record your findings.
(Your looking for changes in color of the apple slices in distilled water, lemon juice and any catechol oxidase activity.)

Part B: Temperature on Enzyme Activity

1.Obtain 4 test tubes. Label them 1-4
2.Add 4 ml of homogenized beans to each tube
3.Incubate tubes 1 & 3 at temp A (ICE water 7 degrees C) and tubes 3 & 4 at temp B (Body temperature 37 degrees C) for 10 minutes.
4.Mix each tube, then take out 1 drop of each tube with a coffee stick and place on a glucose test strip. wait 30 seconds, and read test strip using color scale. Record Data.
5. Use a clean pipet to add 2 ml of Beano to tubes 1 & 3. With another clean Pipet add 2 ml of distilled water to tubes 2 & 4. Mix.
6.Put tubes 1 & 3 in the Temp A water bath and tubes 3 & 4 in temp B water bath.Begin timing.
7. After 5 minutes, mix each tube slightly with coffee stick and then take a drop from each tube. Check Glucose Concentration.
8.After 5 additional minutes (10 minutes total) repeat step 7.
9. After 5 additional minutes (15 minutes total) repeat step 7.
10. After 5 additional minutes 20 minutes total) repeat step 7. Check Glucose for all 4 tubes.
11. Collect Data and record. Draw or Label in Chart.
12. Independent Variable: Temperatures ICE water/ Body temperature
Dependent Variable: Glucose concentration strip.
Constants: Beano, Beans and Distilled water

Part C: Substrate on Enzyme Activity

1. Label 6 test tubes

More about Enzyme Activity

Get Access